欢迎访问硅基纳米生物技术课题组(Sense Group) 中文 S
 
 
当前位置:首页>新闻动态>课题组王会、姜享旭等同学在Analytical Chemistry发表论文
新闻动态

课题组王会、姜享旭等同学在Analytical Chemistry发表论文

发布日期:2014-07-23


题目:  A Hairpin DNA-Assisted Silicon/Silver-Based Surface-Enhanced Raman Scattering Sensing Platform for Ultrahighly Sensitive and Specific Discrimination of Deafness Mutations in a Real System


作者:Hui Wang,(共同第一作者) Xiangxu Jiang,(共同第一作者) Xing Wang,(共同第一作者) Xinpan Wei, Ying Zhu, Bin Su, Yuanyuan Su, Sudan He,* and Yao He,*


单位:
Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou 215123, China

Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital,Soochow University, Suzhou 215123, China

摘要:

Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool for ultrahighly sensitive detection of analytes. In this article, we present a kind of silicon-based SERS sensing platform made of hairpin DNA-modified silver nanoparticles-decorated silicon wafer (AgNPs@Si). In particular, the AgNPs@Si with a high enhancement factor (EF) value of ~ 4.5 × 107 is first achieved under optimum reaction conditions (i.e., pH = 12, reaction time = 20 min) based on systematic investigation. Such resultant AgNPs@Si is then employed for construction of a silicon-based SERS sensing platform through surface modification of hairpin DNA, which is superbly suitable for highly reproducible, multiplexed and ultrasensitive DNA detection. A detection limit of 1 fM is readily achieved in a very reproducible manner along with high specificity. Most significantly, for the first time, we demonstrate that the silicon-based SERS platform is highly efficacious for discriminating deafness-causing mutations in a real system at the femtomolar level (500 fM), which is about 3-4 orders of magnitude lower than that (~ 5 nM) ever reported by conventional detection methods. Our results raise the exciting potential of practical SERS applications in biology and biomedicine.




影响因子:5.695


分区情况:2区

相关链接: http://pubs.acs.org/doi/abs/10.1021/ac501675d

 
首页
研究领域
研究成果
组内成员
新闻动态
组内事务
联系我们
 
友情链接

版权所有©2013 何耀教授课题组(Sense Group) 苏州大学功能纳米与软物质研究院(FUNSOM)

地址:江苏省苏州市工业园区仁爱路199号910楼 邮编:215123