欢迎访问硅基纳米生物技术课题组(Sense Group) 中文 English
 
 
当前位置:首页>新闻动态>课题组(曹朝辉)和医学部何苏丹教授课题组合作在 Nanoscale 上发表论文
新闻动态

课题组(曹朝辉)和医学部何苏丹教授课题组合作在 Nanoscale 上发表论文

发布日期:2017-05-03


题目: In vitro cellular behaviors and toxicity assays of small-sized fluorescent silicon nanoparticles


作者:Zhaohui Cao‡, Fei Peng‡(共同一作), Zhilin Hu‡(共同一作), Binbin Chu, Yiling Zhong, Yuanyuan Su*, Sudan He*,and Yao He*


单位:
a.Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China.

b.Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215123, China


摘要:Extensive investigations have been carried out for evaluating the toxicology of various nanomaterials (e.g., carbon- and metal-based nanomaterials), which offer invaluable information for assessing feasibility of nanomaterials-based wideranging applications. In recent years, sufficient efforts have been made to develop fluorescent small-sized silicon nanoparticles (SiNPs) as novel optical material simultaneously featuring strong fluorescence and ultrahigh photostability, providing high promise for myriad biological, biomedical and electronic applications. It is worth pointing out that, despite non- or low-toxicity of silicon, sufficient and objective toxicology evaluation of SiNPs is urgently required in both in vitro and in vivo level. However, there currently exists scanty information about intracellular behaviors of the SiNPs, particularly the underlying mechanism of entry into cells and intracellular fate. Herein, we give a report aimed at determining the uptake and intracellular transport of SiNPs of ca. 4 nm diameter. Taking advantages of strong and stable fluorescent signals of SiNPs, we reveal that these small-sized SiNPs accumulate at the plasma membrane prior to internalization, and are further internalized predominantly by clathrin-mediated and caveolae-dependent endocytosis. After endocytosis, the SiNPs are localized in early endosomes in short time (~ 1 h), while up to 24-h incubation the SiNPs are mainly transported to lysosomes in a microtubule-dependent way; and interestingly, to a smaller extent are sorted to Golgi apparatus. Moreover, we demonstrate that there are no toxic effects of SiNPs on cell metabolic activity and integrity of plasma membrane.

影响因子:7.76


分区情况:1区

链接: http://pubs.rsc.org/-/content/articlelanding/2017/nr/c7nr00530j#!divAbstract

 
首页
研究领域
研究成果
组内成员
新闻动态
组内事务
联系我们
 
友情链接

版权所有©2013 何耀教授课题组(Sense Group) 苏州大学功能纳米与软物质研究院(FUNSOM)

地址:江苏省苏州市工业园区仁爱路199号910楼 邮编:215123