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Paper published in Nanotechnology by Xiangxu Jiang et. al., cooperating with Prof. Kai Yang's group

Date released: 2017-02-22

Title:Plasmon-enhanced fluorescence imaging with silicon-based silver chips for protein and nucleic acid assay

Authors:Bing Yuan,* Xiangxu Jiang, Chu Yao, Meimei Bao, Jiaojiao Liu, Yujiang Dou, Yinze Xu, Yao He,* Kai Yang,* Yuqiang Ma

1. Coll Phys Optoelect & Energy, Ctr Soft Condensed Matter Phys & Interdisciplinar,Soochow University, Suzhou 215123, China

2. Institute of Functional Nano & Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-based Functional Materials & Devices, Soochow University, Suzhou 215123, China

Abstract:Metal-enhanced fluorescence shows great potential for improving the sensitivity of fluoroscopy, which has been widely used in protein and nucleic acid detection for biosensor and bioassay applications. In comparison with the traditional glass-supported metal nanoparticles (MNPs), the introduction of a silicon substrate has been shown to provide an increased surface-enhanced Raman scattering (SERS) effect due to the coupling between the MNPs and the semiconducting silicon substrate. In this work, we further study the fluorescence-enhanced effect of the silicon-supported silver-island (Ag@Si) plasmonic chips. In particular, we investigate their practical application of improving the traditional immunoassay such as the biotin-streptavidin-based protein assay and the protein-/nucleic acid-labeled cell and tissue samples. The protein assay shows a wavelength-dependent enhancement effect of the Ag@Si chip, with an enhancement factor ranging from 1.2 (at 532 nm) to 57.3 (at 800 nm). Moreover, for the protein- and nucleic acid-labeled cell and tissue samples, the Ag@Si chip provides a fluorescence enhancement factor of 3.0–4.1 (at 800 nm) and a significant improvement in the signal/background ratio for the microscopy images. Such a ready accommodation of the fluorescence-enhanced effect for the immunoassay samples with simple manipulations indicates broad potential for applications of the Ag@Si chip not only in biological studies but also in the clinical field.



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