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Paper published in Analytical Chemistry by Huayi Shi et.al

Date released: 2017-09-08

Title:Reusable Silicon-Based Surface-Enhanced Raman Scattering Ratiometric Aptasensor with High Sensitivity, Specificity and Reproducibility

Authors:Huayi Shi, Na Chen(co-first author), Yuanyuan Su, Houyu Wang,* and Yao He*

Institute of Functional Nano & Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China

Abstract:Rapid, sensitive and accurate detection of adenosine triphosphate (ATP), the primary energy molecule, is critical for the elucidation of its unique roles in cell signaling and many cellular reactions. Up to date, major challenge is still remaining for fabricating surface-enhanced Raman scattering (SERS) aptamer sensors (aptasensors), suitable for accurate and reliable quantification of ATP. Herein, we develop the ratiometric silicon SERS aptasensor for ATP detection, which is made of uniform silver nanoparticles (Ag NPs)-modified silicon wafer (Ag NPs@Si), followed by the functionalization with double-stranded DNA (dsDNA I). The dsDNA I is formed by aptamer and its complementary DNA (cDNA), which contains two independent segments (e.g., 5’-Cy3-labeled DNA-C1, 3’-ROX-labeled DNA-C2). In the presence of ATP, ROX-DNA-C2 is dissociated from dsDNA I due to the formation of aptamer/ATP complex, leading to the attenuation of ROX signals; and meanwhile, Cy3 signals remain constant ascribed to the formation of dsDNA II caused by the supplementation of aptamer. As a result, ratiometric signals of the ratio of Rox intensity to Cy3 intensity (IROX/ICy3) can be achieved. Of particular significance, the developed ATP aptasensor features excellent reproducibility (e.g., the RSD is less than ~4%, comparable or superior to that of previously reported aptasensors), ultrahigh sensitivity (e.g., the detection of limit (LOD) reaches 9.12 pM, lower than that of other reported ATP SERS aptasensors), as well as good recyclability (e.g., ~9.3% of RSD values of ratiometric signals within 3 cycles).



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